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The Procedure – A detail explanation about the production of the ELISA test and finally your test results

Below is a detailed explanation of the IgG test in detail, right from the production of the ELISA test to the processing of your samples and then finally the test results.

Production – ImuPro test plates

Once we receive the empty micro titer plates proteins are extracted from 270 foodstuffs. It is very important to ensure that the food extracts reflect the nature of the proteins that are involved in the type III allergy reaction.

These 270 foods are then applied to empty plates – each allergen extract is then poured into separate wells on the plate keeping in mind not to pool the allergen extract because if we did so then we wouldn't know which food has caused the reaction and this would not give a clear picture as to which food needs to be restricted from your diet. Once this is done the plates are then sent to a diagnostic laboratory and are now ready to process your sample.

Step 1

Pipetting of your sample

The sample IgG antibodies serum is then processed in the laboratory and then applied to the entire plates, placing a portion into each well.

The IgG antibodies that are present in the serum are left for around 90 minutes to allow it to bind with the food antigens. These antibodies will only bind to their respective counterpart allowing us to see which foodstuff has produced these antibodies against.

Step 2

Washing – micro titre plate

In the second step, the plates are then washed and only the antibodies which are captured remain in the well.

Step 3

Pipetting – antibody to IgG

A substance is then applied to the plate which then enables us to see the binding of an antibody to IgG.

This second antibody will then detect the IgG that are binding to the foodstuffs present in the wells of the plate and are capturing the IgG from your sample. The antibody carries an enzyme that produces a colour reaction. The new antibody binds to the ends of the IgG antibodies present during a reaction time of 90 minutes.

Step 4

Washing – micro titre plate

The plate is washed again and only the antibodies to IgG that have bound to the food proteins remain in the well.

Step 5

Colour reaction

A staining colourless solution is then added. During a reaction time of 60 minutes, the enzymes trigger a colour reaction due to the antibodies present. The intensity of the colour reaction will depend on the quantity of antibodies in a well.

The amount of IgG antibody titres can be determined due to the intensity of this colour reaction that is proportional to the quantity of IgG antibodies from your sample.

Step 6

Evaluation and finally your test results

The results are then evaluated and the foodstuffs are then placed into three different groups – not elevated, elevated and highly elevated and your results are then prepared according to these findings.